10ml of each strain was added as inoculums in 500ml flask contain 240ml synthetic wastewater, the flasks were
incubated in the rotary shaker at 170rpm at 37°C . Samples were collected ever two hours to monitor bacterial
growth at 600nm and biodegradation rates. The bacterial growth and biodegradation rates were monitoring by
measuring the optical density and TOC.
3.3. TOC Determination
TOC analysis were carried out using Hach Lange Cuvette test (LCK 386) as follows: 1.0ml of sample was
pipetted into the digestion cuvette, which then inserted open in the TOC-X5 shaker for five minutes. When the
sample preparation is completed, the indicator cuvette with membrane double cap was tightly screwed on the TOC
cuvette, and then digested at 100 °C for 120 min, then allowed to cool, cleaned and evaluated
3.2. Experiment study
Commercial strain of SludgeHammer and local isolated strains of bacteria identified as Bacillus spp., Bacillus
subtilis, Bacillus laterosponus, Bacillus agri, Pseudomonas aeruginosa, and Pseudomonas putida were used.
10ml of each strain was added as inoculums in 500ml flask contain 240ml synthetic wastewater, the flasks were
incubated in the rotary shaker at 170rpm at 37°C . Samples were collected ever two hours to monitor bacterial
growth at 600nm and biodegradation rates. The bacterial growth and biodegradation rates were monitoring by
measuring the optical density and TOC.
3.3. TOC Determination
TOC analysis were carried out using Hach Lange Cuvette test (LCK 386) as follows: 1.0ml of sample was
pipetted into the digestion cuvette, which then inserted open in the TOC-X5 shaker for five minutes. When the
sample preparation is completed, the indicator cuvette with membrane double cap was tightly screwed on the TOC
cuvette, and then digested at 100 °C for 120 min, then allowed to cool, cleaned and evaluated
3.2. Experiment study
Commercial strain of SludgeHammer and local isolated strains of bacteria identified as Bacillus spp., Bacillus
subtilis, Bacillus laterosponus, Bacillus agri, Pseudomonas aeruginosa, and Pseudomonas putida were used.
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