and designing of new xylanases with higher efficiency
and specificity via genetic manipulation has become a
major focus of researchers in this field.
Recently, a bacterial strain was isolated from dewrotten
ramie in our lab and was identified by morphology,
biochemical and physiology characters primarily.
The results of traditional method showed that the strain
had very similarity with Bacillus subtilis. This strain,
named B10 has been shown to produce xylanase with
higher extracellular activity, about 485.7 U/ml, and
could be used to remove the gummy material from plant
fibers (Xiao et al., 2004). In this report, the strain B10
was further identified by PCR amplification 16S rDNA,
and we described the cloning, sequencing, expression
and characterization of the B. subtilis strain B10-derived
xylanase gene in E. coli.