The yield of DNA extracted was measured using a UV spectrophotometer (UV-1601 Shimadzu, Kyoto Japan) at 260 nm. The purity of DNA was determinedby calculating the ratio of absorbance at 260 nm to that of 280 nm. DNA concentration and purity was also determined by running the samples 1.0% Agarose gel, based on the intensities of band when compared with 250 bp DNA marker from Chromous Biotech (Bangalore India).