2.5. Seed germination
The seeds corresponding to each treatment were germinated at
25 C for 5 days in darkness, using a climatic cabinet (ASL Snijders
Sci. International S.L., Tiburg, Holland), previously sterilised by ultra
violet radiation (Double UV lamp Unit, Model 022.9120, Camag,
France) at 254 nm for 16 h. Seeds were sprinkled with distilled
water every 12 h. The percentage of germination was determined
by counting the number of germinating seeds after the germination
period. Twenty five seeds were germinated for each treatment,
and germinations were performed in duplicate.
.