Compound separation was operated by High Performance
Liquid Chromatography (HPLC) coupled with an on-line antioxidant
capacity detection system. The system consist in a Dionex
Ultimate Autosampler WPS3000FC, a Dual Gradient Pump 3600 SD,
a Diode Array Detector 3000 (RS) and a Variable Wavelength Detector
3100. The chromatographic column was an ACE C18-PFP
(Advanced Chromatography Technologies LTD, Scotland,
250  4.6 mm; 5 mm). A binary solvent system was used at a flow
rate of 1 mL/min with A: Milli-Q water/Trifluoroacetic acid (99.9/
0.1) (v/v) and B: Acetonitril, applying the gradient presented in
Fig. 1. DAD detection was operated between 200 and 700 nm.