The number of Mycoplasma-specific IFN-g SC were
determined by using an AID1 ELISpot Reader and AID1
ELISpot software v. 6.0 (Autoimmun Diagnostika, Strassberg,
Germany). As a positive control, 1 105 PBMC/well
were incubated with PHA (10 mg/ml); as a negative
control, 8 105 PBMC were incubated without antigen.
The background values were subtracted from the