After 1 and 2 h an aliquot (1 mL) of each
solution was taken and subjected to serial dilutions. To determine
the count of viable cells, 1 mL of the dilution was inoculated
through deep seeding in acidified MRS agar and incubated anaerobically
at 37 C for 72 h (Liserre, Re, & Franco, 2007; Mishra &
Prasad, 2005).