Pectinex-catalyzed and dilute acid saccharification of lipid-extracted residual biomass from Chlorella sp. KR-1 generated
19.1 and 24.4 g/L of reducing sugars, respectively. The dilute acid hydrolysate from the lipid-extracted residual biomass was converted into bioethanol by SHF with a yield of 0.4 g ethanol/g fermentable sugar. The bioethanol yield based on the lipid-extracted residual biomass was 0.16 g ethanol/g biomass. In this study, we demonstrated the use of lipid-extracted residual biomass for improved economic feasibility of microalgae biomass for the production of biodiesel and bioethanol.