There are few biosensor researches referred to Aβ detection, the study based on electrochemical immunosensor used screen-printed carbon electrode as an interface and detected the signal by cyclic voltammetry but it is always necessary to have a specialist and expensive devices. The lateral flow biosensor overcomes those problems and suitable for Aβ measurement. Because of Alzheimer’s disease test require quantitative assessment, the combination of Aβ lateral flow test strip, digital camera and image processing program as analyzing system has been developed.