Substrate utilization tests were performed by
using ISP9 (Shirling & Gottlieb, 1966) as the basal medium.
Each substrate was added at a final concentration of 0.2%
(w/v) according to Ka¨mpfer et al. (1991). Aerobic production
of acids from carbohydrates was determined by using
API 50CH test kits (bioMe´rieux) with API 50CHB/E
medium (bioMe´rieux) in combination with 96-well microtitre
plates as described by Schmidt et al. (2012)