2.3.2. Carotenoids analysis by high performance liquid
chromatography (HPLC)
The composition of solvents and the isocratic conditions used
were described previously by Olives Barba et al. (2006). Analysis
was performed using Shimadzu LC-20AC pumps, a SPD-M20A
diode array detector, and a LUNA C-18 column (4.6 250 mm
i.d., 5 lm). The mobile phase consisted of methanol (solvent A)/
acetonitrile + triethylamine (TEA) 9 lM (solvent B) 90:10 at a flow
rate of 0.9 ml/min. The column temperature was 30 C and the absorbance was read at 475 nm