3.2. Practical Considerations in Induced Crop Mutagenesis
Mba et al. [39] provided the protocols for the induction of mutations in seed and vegetatively propagated plants, based on procedures validated for rice and cassava, respectively. The protocols were for the physical mutagen (gamma rays generated by a cobalt-60 source) and the chemical mutagen (ethyl methane sulfonate, EMS). The authors [39] listed the required equipment and reagents and described the sequential procedures, the pre-treatment handling of the propagules, the mutagenic treatments, post-treatment handling of the materials; and the methodologies for the collection and analysis of data. In general, the steps to the induction and detection of mutants vary between seed and vegetatively propagated crops but would nonetheless involve the following considerations:
3.2. Practical Considerations in Induced Crop Mutagenesis Mba et al. [39] provided the protocols for the induction of mutations in seed and vegetatively propagated plants, based on procedures validated for rice and cassava, respectively. The protocols were for the physical mutagen (gamma rays generated by a cobalt-60 source) and the chemical mutagen (ethyl methane sulfonate, EMS). The authors [39] listed the required equipment and reagents and described the sequential procedures, the pre-treatment handling of the propagules, the mutagenic treatments, post-treatment handling of the materials; and the methodologies for the collection and analysis of data. In general, the steps to the induction and detection of mutants vary between seed and vegetatively propagated crops but would nonetheless involve the following considerations:
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