Pollen would act as a gametic screen for euploids, as it is less tolerant of chromosomal abnormalities than the female gametophyte. Microspore culture was used to select for euploid plants within the SI BC2F1 for subsequent backcrossing. When the male gamete alone was used to produce the next generation through microspore culture, the progeny of the majority of lines fit the 1 :1 ratio. Those DH progenies that did not fit the 1 :1 ratio were progenies with an over-representation of the SC class. This result is consistent with aneuploidy in BC2 SI plants. Microspore culture has been shown to select for euploid gametes