Amplicons were analyzed in 2% agarose gel electrophoresis
using a 1xTAE buffer at 100 V, followed by ethidium bromide
staining (0.5 mg ml1). Bands were visualized under Ultra Violet
(UV) light using a Gel Doc XRþ system (Bio-Rad Laboratories, USA).
Molecular sizes were estimated by comparison with the DNA
standard GeneRuler™ 100 bp or 50 bp DNA ladder (Thermo Scientific,
USA).
94 C, 30 s at 60 C (53 C for ITS) and 20 s at 72 C, and a final
extension step at 72 C for 7 min