Fermentation was carried out in 250 ml plugged Erlenmeyer flasks, each containing 50 mL of medium and inoculated with volumes of pre-inoculum corresponding to 0.8 O.D. After pre-inocula homogenization by ULTRA-TURRAX®, The inocula were incubated at 28°C on rotary shaker at 225 rpm for fifteen days.