The final gradient using the Kinetex 5 mm C18 100 A˚ was as follows: 0% B isocratic for 8 min, 0–50% B in 20 min, 50–100% B in 7 min, back to 0% B in 3 min, and 0% B isocratic for 2 min.
The final gradient using the Kinetex 5 mm C18 100 A˚ was as follows: 0% B isocratic for 8 min, 0–50% B in 20 min, 50–100% B in 7 min, back to 0% B in 3 min, and 0% B isocratic for 2 min.