BRAIN HEART INFUSION AGAR (BHIA) is a solid medium rich in nutrients, suitable for the cultivation of several
fastidious strains of bacteria, fungi, and yeasts. Brain Heart Infusion Agar is used for the cultivation of a wide variety of fastidious microorganisms such as streptococci, meningococci and pneumococci. BHIA is recommended in Standard Methods for water testing and in antimicrobial susceptibility tests. The nutritionally rich base of Beef heart and Calf brain infusions and Peptone mixture provide nitrogen, vitamins, minerals and amino acids that supports the growth of a variety of microorganisms. Disodium phosphate acts as a buffer.Dextrose is the fermentable carbohydrate providing carbon and energy.Sodium chloride maintains the osmotic balance. Bacteriological agar is the solidifying agent