Methods: In silicoMHCclass II binding predictionwas performed
with NetMHCIIpan 2.0 (peptide length, 15; 1-mer offset) and the
most abundant class II alleles in the North American population
andwith an in vitroMHCclass II peptide reporter assay performed
in parallel, which used synthetic 15-mer peptides offset by 5 mer
spanning the protein.