High performance liquid chromatography
Extracts of fresh and different drying processed soursop leaves with fixed solvent extraction (solvent selection based on TBC, TVC and TO ability) will be analysed of using reverse-phase HPLC with a phenyl column.
A 15 min linear gradient from 5% to 100% MeOH will be added to elute samples at 1 ml/min.
Mobile phase will be acidified with 0.1% trifluroacetic acid for better resolution.
Elution will be monitored at 254 nm.