γ-Oryzanol content in RBO was determined according to the method of Mezouari and Eichner (2007) with a slight modification. Briefly, rice bran oil was mixed with n-heptane to proper dilution. γ-Oryzanol content in the mixture of RBO and n-heptane was determined by a UV spectrometer at 315 nm (Shimadzu, Japan). A standard curve of γ-oryzanol was prepared and used for the calculation of the concentration of γ-oryzanol, which was expressed as g/100 g sample.