The effects of mechanically debone poultry meat (MDPM) and levels of collagen fibers on comminute, cooked sausage quality characteristics were investigated using the central composite rotate design of response surface methodology (RSM). Use of collagen fiber as an additive affected the sausage characteristics, but the effect depended on the amount of the MDPM used. While MDPM additions resulted in higher cooking loss and darker and redder frankfurters, the addition of collagen fibers improved cooking yields and contributed to the lightness of the final product. Higher collagen fiber content was also accompanied by a significant increase in frankfurter hardness regardless of the MDPM content. Use of collagen fibers counter the negative effects of MDPM on sausage quality attributes, especially on cooking yields and final product color.