optimized to 467 mg L−1in the fed batch (Seetha Ram et al., 2014). These laststudies indicate the potential use of intein for protein purificationon both small and large commercial scales. In fact, the simplic-ity afforded by this method, which requires fewer isolation stepsand a reduced amount of reagents, suggests that inteins can beused to decrease the cost of producing recombinant proteins (Bankiand Wood, 2005).