Samples taken from the strain verification in hydrolysate liquor were analyzed by HPLC using a BioRad Aminex HPX-87H column. Glucose, ethanol, and acetic acid concentrations were measured with a refractive index detector, at 45 °C, and 5 mM H2SO4 as eluent in a flow rate of 0.6 mL/min. Furfural, HMF and syringaldehyde were measured with a UV detector at 210 nm, 65 °C, and a mixture of 90:10 5 mM H2SO4:acetonitrile as eluent in a flow rate of 0.6 mL/min.