According to the stoichiometry of alcoholic fermentation, the sum
of the analytical data related to the concentrations of unconverted
sugars, accumulated glycerine and half of ethanol formed did not
vary significantly with time, assuming values very close to the initial
concentration of sugar used (see M= molar balance; Figures 1a,1b
and 3a,3b). As a consequence, a possible significant accumulation of
intermediates can be ruled out and this relation can be written: