there is no evidence to indicate
whether the resulting embryos, when used for embryo transfer, can lead to the transmission
of BVDV to recipients or offspring. For this experiment, semen from a bull
persistently infected with BVDV (105 50% tissue culture infective doses/mL NY strain) was
used for insemination (two times at estrus) of BVDV-seronegative, superovulated cows
(N ¼ 35).