malt extract for 2 days at 30°C with standing. The grown cells were transferred into 10 ml of the juice, without supplementation of extra nitrogen sources, in 15 ml-plastic tubes with 0.1%-inoculation. The lids of the tubes were loosely tightened to avoid evaporation of the ethanol produced and to remove the generated carbon dioxide gas. After 5 days of cultivation at 30°C with standing, the ethanol production, sugar consumption, yeast growth, and pH were measured. As shown in Table 1,