In order to study the antifungal effects of the CS-BA nanogel encapsulated
T. vulgaris essential oils in vivo, the fruit were first
washed with detergent, and were sprayed with 2 ml of sporecontaining
solution (150 spores/ml). Subsequently, the fruits
were sprayed with different concentrations of the CS-BA nanogel-encapsulated
T. vulgaris essential oils ranging between
0 and 900 mg/l and the fruit were stored in dark for one
month at ambient temperature. Then, the spores were collected
by rinsing the fruits with 25 ml sterilized distilled water followed
by centrifugation at 3000 g for 10 min. Finally, the
culture media were inoculated by the collected spores. After 5
days, the developed colonies were counted. The experiment
was conducted in triplicates with 6 fruits designated to each
replicate