The 3-item CNS was found to be reliable and valid for both cultural groups, while the HEPCON scale was found to be reliable and valid only for Japanese respondents, for whom higher neophobia hindered the acceptance of both novel and familiar foods. Among Japanese consumers, low prior knowledge and stronger health-oriented attitudes were associated with acceptance of familiar albacore dishes, whereas high prior knowledge and stronger health-oriented attitudes were associated with lower acceptance. Willingness to try novel albacore dishes was marginally enhanced when written information on health benefits of omega-3 FA was provided or when pleasure-oriented attitudes were strong.