Determination of light necessity for basidiomata primordia
induction
Three strains were selected for production assay: ICFC 47/99,
ICFC 751/12 and 748/12. 25 × 40 cm polypropylene bags (30 m
thick) were filled with 100 g of Eucalyptus sawdust; moisture was
adjusted to 70% of humidity using tap water and then sterilized
at 120 ◦C, 1.2 psi for 2 h. Once bags, reached room temperature,
they were inoculated with 5 g of spawn prepared with strain ICFC:
748/12. Then bags were moved to incubation room and maintained
at 25 ◦C in the dark for 60 days. For this test we used an
80 × 40 × 30 cm fishbowl divided in to two equal compartments.
Glass walls of one of them and the upper part were coated with
opaque black polyethylene plastic film to avoid light from coming
in and to keep it completely dark, the other compartment was covered
with a transparent polyethylene plastic film (Fig. 1). A plastic
tube was placed in both compartments to supply fresh air produced
by a fishbowl aerator.
After spawn running, plastic bags were removed and four blocks
were then placed in each compartment. Manual watering was daily
carried out with a sprayer. The fishbowl was placed in the production
room maintained at a temperature of 20 ◦C and with a
photoperiod of 9 h light and 15 h dark. After 20 days primordia
formation,form and color were evaluated. Color notations in parentheses
are from The Online Auction Color Chart Company, 2010.