Spore suspensions were heat activated (80 C,
15 min), and incubated anaerobically at 30 C in TY broth plus Lalanine
(100 mM) and L-lactate (50 mM) to observe emergence and
outgrowth. Samples (500 ml) were taken at 30 min intervals for 5 h,
centrifuged (4000 g, 2 min, 4 C), and the remaining pellet prepared
for SEM.