added to a glass tube containing 5 ml of sterile distilled water and
ultrasonicated (90 U) for 10 min. Serial dilutions were prepared and
conidia were counted using a hemacytometer.
The biological variables analysed for each treatment and replication were: inhibition of mycelial growth (IMG) expressed as
the percentage ratio of the diameter of mycelial growth in each
treated plate relative to the diameter of growth on the control
treatment, standardized area under the fungal growth progress
curve (SAUGPC) calculated by the trapezoidal integration method
standardized by the duration fungal growth in days (Campbell and
Madden, 1990) and fungal sporulation (conidia/mm2). Analysis of
variance (ANOVA) was performed to SAUGPC, angle-transformed
percentage IMG, and transformed [log (conidia/mm2 + 1)] sporulation. Means were compared using Fisher’s protected least
significant difference tests (P= 0.05).