Enzymatic hydrolysis of the regenerated cotton cellulose
Wet cotton cellulose regenerated from 1 g cotton cloth was placed in 20 mL of 50 mM citrate buffer (pH 5.0)
containing 3.3 U/mL cellulase and incubated at 50 C with shaking at 80 rpm.
The reaction was monitored by withdrawal of samples from the supernatant
and measuring the amount of soluble reducing sugars by using an assay based on 3,5 dinitrosalicylic acid (DNS)with D-glucose as the standard.