In view of the observed increased stability, the three lipases immobilized on epoxy functionalized silica (CALB, RML and TLL)
were used for transesterification reactions. No transformation occurred in the control media. The behavior of the preparations
in the reaction between canola oil and increasing methanol concentrations (oil/methanol molar ratios from 1:3 up to 1:9) was
investigated. Since the stepwise addition of methanol is the most common strategy in earlier studies, the 3-stepwise methanol feeding method was utilized.