During our studies on the distribution of anaerobes in
flowers, we have isolated several species of lactic acid
bacteria (LAB) from mountain flowers in Japan. We
recently reported the isolation of a novel LAB, Lactobacillus
floricola, from a mountainous area (Kawasaki et al., 2011).
L. floricola showed quite low 16S rRNA gene sequence
similarity to known LAB, and also showed a narrow range
of carbohydrate utilization, using only glucose and fructose.
Here, we report the isolation of further isolates that
exhibit low 16S rRNA gene sequence similarity to known
LAB and show a narrow range of carbohydrate utilization.
During the course of our investigations of anaerobes in
flowers from several mountainous areas in Japan since
2006, the novel species described in this study has been
isolated only from the area of Oze National Park.
Flowers were collected from Oze National Park in 2008–
2009. Flower samples were collected using autoclaved