Fig. 2 – Cytotoxicity of luteolin and luteolin glycosides in LPS-stimulated RAW 264.7 macrophages. Cells were pre-incubated with either 50 or 100 M luteolin (Lut), luteolin 7-O-β-glucopyranoside (L7OG), isoorientin (IsoO), cassiaoccidentalin B (CasB) or orientin (O) for 1 h and then stimulated with LPS for 24 h. Cell viability was assessed using MTT assay and results expressed relatively to LPS-treated cells. Each value represents mean ± SEM of at least 3 independent experiments (***p < 0.001, when compared to LPS).