Statistical significance was determined by comparing the relative fluorescence units (RFU) at the 60 minute time point between the DMSO solvent control and a studied compound. Baseline RFU (lowest RFU value exhibited by DMSO control) from each trial was subtracted from all RFU values (60 minute time point) for that given trial and each group containing a studied compounds was represented as percentage of DMSO control group. A paired t-test was used to compare the studied compound to the DMSO control based on three trials. A p value of less than 0.05 was considered significant.