Total phenol contents of the phenolic extracts were determined
by the Folin–Denis method. The Folin–Denis reagent was prepared
by the method of Perry, Burgess, and Glennie (2001). The absorption
measurements were performed on a UV–visible spectrophotometer
(Optima SP-3000 plus, Tokyo, Japan) at 745 nm. The
same procedure was followed to analyse total phenols in WK and
CT using 1.0 g of each of the component for the extraction of phenolic
substances. Total phenols in CW were directly determined by
the same colorimetric method.