Limit of detection and limit of quantification
The analysis was carried out at decreasing concentrations to determine the minimal concentration with a signal-to-noise ratio of 3:1.
Under the experimental conditions, the LOD value was 0.023 μg/mL whereas LOQ values were 0.07 μg/mL respectively. In addition, the analyte peak of the LOQ samples was identifiable, discrete, and reproducible with accuracy within ≤ 20% and a precision of ≤ 20%(Figures 2 and 3).
Table 1 shows the results of statistical analysis of the experimental data, such as the slope, the intercept, and the correlation coefficient obtained by the linear square treatment of the result along with the standard deviation of the slope (Sb) and intercept (Sa).