Following 21 days postmortem aging, each muscle was trimmed to remove all visible or external connective tissue and exterior fat. In addition, the gluteus medius was removed from the strip loin. The biceps femoris, gluteus accessories, and gluteus profundus were removed from the top sirloin butt. The gracilis, adductor, pectineus, and sartorius muscles were removed fromthe top round. The resulting muscles were fabricated into 2.5 cm-thick steaks. A steak from each subprimal was designated for proximate analysis, Warner–Bratzler shear force (WBSF), and fatty acid analysis in a rotational order throughout the muscle to eliminate any bias. All remaining 2.5-cm steaks were portioned into smaller piecesmeasuring approximately 5 cm×5 cm. Steaks for proximate analysis,WBSF, fatty acid analysis and consumer testing were packaged, and frozen (−10 °C) until sorting.Once frozen the samples were sorted into a predetermined balanced cook order. Cooking groups (n=48) of 10 steak pieces fromeach treatmentwere packaged and stored in the absence of light at−10 °C until consumer evaluations