In the present work, the effect of co-culture on bacteriocin production by selected lactic acid bacteria
(LAB) was studied. Thus, the co-culture of two bacteriocin-producer strains, Lactobacillus plantarum NC8C
and Enterococcus faecium 6T1a, resulted in a significant reduction or even suppression of bacteriocin
production by both strains. The phenomenon was not restricted to these two strains since we have
further proved that it was more general and also occurred in several other co-cultures with LAB
isolated from different food fermentations. This decrease in bacteriocin production was confirmed to be
non cellecell contact-dependent by using a two-compartment system in which the microorganisms
were separated by a 0.2-mm-pore-size membrane. In addition, cell-free supernatants from certain cocultures
were able to suppress bacteriocin production in other strain cultures. In light of these findings,
we hypothesize that such interactions between microorganisms should be taken into account to
improve the development of bacteriocin-producing starter cultures used in food fermentations in order
to avoid a negative effect on the quality of the fermented product
In the present work, the effect of co-culture on bacteriocin production by selected lactic acid bacteria(LAB) was studied. Thus, the co-culture of two bacteriocin-producer strains, Lactobacillus plantarum NC8Cand Enterococcus faecium 6T1a, resulted in a significant reduction or even suppression of bacteriocinproduction by both strains. The phenomenon was not restricted to these two strains since we havefurther proved that it was more general and also occurred in several other co-cultures with LABisolated from different food fermentations. This decrease in bacteriocin production was confirmed to benon cellecell contact-dependent by using a two-compartment system in which the microorganismswere separated by a 0.2-mm-pore-size membrane. In addition, cell-free supernatants from certain cocultureswere able to suppress bacteriocin production in other strain cultures. In light of these findings,we hypothesize that such interactions between microorganisms should be taken into account toimprove the development of bacteriocin-producing starter cultures used in food fermentations in orderto avoid a negative effect on the quality of the fermented product
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