According to the study of Soylu he study ab
.Antifungal activities of essential oils obtained from aerial parts of aromatic plants, which belong to the Lamiacea family such as origanum, lavender and rosemary
For determination of contact effects, the essential oils were dispersed as an emulsion in water using ethanol and Tween 20 and added to PDA immediately before it was emptied into the glass Petri dishes The concentrations tested were 0.4 to 25.6 μg/ml.
For determination of volatile phase effects, glass Petri dishes were used. The Petri dishes inoculated as described above at different concentrations of essential oils were added to sterile filter papers and placed on the inner surface of the lid of Petri dishes to obtain final concentrations of 0.05 to 1.6 μg/ml air.
The volatile and contact phase effects of different concentrations of essential oils on the mycelial growth of B. cinerea are shown in Fig. 1. All essential oils were found to inhibit the growth of B. cinerea in a dose-dependent manner. Volatile inhibitory effect of essential oils on mycelial growth was greater than contact inhibitory effect (Fig. 1). In the contact phase of essential oils, relatively higher concentrations were required to inhibit mycelial growth as shown in Fig. 1.