Liverworts are non-vascular cryptogamic plants with wide geographic distributions due to their capacity to disperse their spores over long distances, although they have no epidermal cuticle, sensitive to external environmental conditions, found only in very specific microenvironments – and are therefore widely used as bioindicators. We evaluated the floristic composition of liverworts in localities located in different Atlantic Forest vegetation types in southeastern Brazil to address the following questions: Is the liverwort flora distributed uniformly or randomly, or does it demonstrate environmental niche restrictions at regional scales?