When
direct examination showed that the fungus was
sporulating on the berry, spore masses were
picked off with a sterilized wire loop and
streaked on the surface of water agar. After
incubation overnight (28-30°C), single germinated
spores were picked up with a sterilized
needle and transferred to PDA. Pure cultures
were stored in sterilized water in Eppendorf
tubes at 6°C (Abang, 2003). Cultures are
deposited in the culture collection of Mae Fah
Luang University, BIOTEC Culture Collection
(BCC), and CBS.