Range-finding studies were run to determine the appropriate concentrations of the clove essential oil and its constituent compounds for determining LD50 values. Five concentrations of clove essential oil and its two constituents (eugenol and β-caryophyllene) were selected for adults (0.25–4.00 mg/ml) and nymphs (0.95–15.20 mg/ml). Five concentrations of eugenol acetate were used for adults and nymphs test at the range of 2.35–37.60 mg/ml. Five concentrations of abamectin (0.01–0.16 mg/ml) were used for both the adults and nymphs as a positive control, and acetone alone was used as a negative control. Five replicates were prepared for each dose level.