3.2. Results of Enzymatic Hydrolysis
The expected result of this study is to produce high reduction sugar from hydrolysis process. These results are
obtained by comparing the variation of the concentration of cellulose enzyme used and the temperature during the
process of hydrolysis, as well as during the sampling process of hydrolysis for 24 hours. There were 6 series of
experiments to be done in a series of experiments. The average yield of reduction sugar obtained are presented in
Figure 1 for the hydrolysis at temperature of 40°C and Figure 2 for hydrolysis at a temperature of 50°C. The highest
reduction sugar content was obtained at the hydrolysis for 12 hours. The performance of the enzyme increased start
at 0 to 12 hours of hydrolysis shown by the increased reduction sugar content. However, the sampling of the 18
hours of reduction sugar decreased. It indicated that the cellulase enzymes used only reached a maximum working
until 12 hours.
Decreased levels of reduction sugar after the 12 hours was due to the enzymes used are influenced by time and
temperature. The longer use of the cellulase enzyme hydrolysis process, the enzyme activity decreased as indicated
by decreased levels of reduction sugars (see Figure 1 and Figure 2). Kodri (2013) in Gautam et.al (2011) stated that
the activity of cellulose enzyme will increase at the beginning and begin to decline after the eighth day. In addition, a
decrease in enzyme activity is also due to the temperature factor as a result of this Trichoderma sp type of mold
cannot stand too high temperature. In line with previous studies, Gautam et.al (2011) obtained the highest enzyme
activity in the temperature range of 40-50°C for the production of cellulase enzymes from Trichoderma sp.