The polymer used in this study was a styrene-acrylic resin (R579; ResinFam Co., Iran) with a pH value of around 9 and Tg (glass transition temperature) of 51 °C, and mean particle size of 220 nm, according to the supplier. A polyethylene emulsion wax was purchased from Kala Kar (EE 95, Kala Kar Co., Iran). Polyaluminum chloride from Merck Company was used as a coagulating agent. All the mentioned materials were used as received.
Coumarin derivatives (e.g., MUG, MUP, DiFMUP) are extensively used as substrates for detecting enzymatic activity in cells, homogenates, and solution.
We offer a range of coumarin-based reagents as well as high-performance fluorophores with improved characteristics for labeling and detection.