An inexpensive zein-lipid mixture was isolated from yellow dent, dry-milled corn. Grease permeation
through zein isolate applied to brown Kraft paper was found to be independent of loading levels at
zein isolate levels above 30 mg/16 in.2. The data shows that water vapor transmission rates depended
on the amount of coating applied. Triacylglycerols were the most abundant lipid in milled corn but
were absent in the zein isolate (perhaps due to hydrolysis by lipases). Zein from the paper was
hydrolyzed enzymatically and the hydrolysis monitored by SDS-capillary electrophoresis. At an E:S
ratio of 1:100 no further increase in the hydrolysate peak occurred after 10 and 30 min for
R-chymotrypsin and pancreatin 8; however, zein and lipid were still present 1 h after hydrolysis
by pancreatin 1