Effect of Temperature and pH on
antimicrobial activity of extracts
Five milliliters of 100mg/ml of acetone extracts
were constituted in test tubes and treated at 4,
30, 60 and 100oC in a water bath for 30minutes
and tested for antimicrobial activity.
To determine the effect of pH, acetone extracts
were treated at pH ranges of 2.5 to 10 using 1N
HCl and 1N NaOH solutions respectively in
series of test tubes for 30minutes. After 30
minutes of treatment, each of the treated
extracts were neutralized (pH 7) using 1N HCl
and 1N NaOH as the case may be, and then
tested for antimicrobial activity.