Antimicrobial assay
The antibacterial and antifungal activity was carried out by following agar well diffusion
method.
[7] Each plate, a single well of 6mm diameter was made using a sterile borer. The test
compound was introduced into the well using a micropipette. The plates were incubated
overnight at 370C. Microbial growth was determined by measuring the diameter of the zone
of inhibition. For each strain a control was also maintained and the experiment was done
three times for confirmation of activity and the mean values are presented.