Isolation of halophilic and halotolerant actinomycetes
Halotolerant and halophilic actinomycetes were isolated from sediment samples in three different
media viz. Starch casein agar (soluble starch 10 g/L; casein powder 1 g/L; sea water
37 g/L; agar 15 g/L; pH 7.2 2), R2A agar (casein acid hydrolysate 0.5 g/L, yeast
extract 0.5 g/L, proteose peptone 0.5 g/L; dextrose 0.5 g/L; soluble starch 0.5 g/L; dipotassium
phosphate 0.3 g/L; magnesium sulphate 0.024 g/L; sodium pyruvate
0.3 g/L; pH 7.2 2) and Inorganic salt starch agar (soluble starch 10 g/L; di-potassium
phosphate 1 g/L; magnesium sulphate 1 g/L; ammonium sulphate 2 g/L; calcium
carbonate 2 g/L; ferrous sulphate 0.001 g/L; manganous chloride 0.001 g/L;
agar 15 g/L; pH 7.2 2) at 4 different salt concentrations, 32, 50, 75 and 100 psu.
Isolation of halophilic and halotolerant actinomycetes Halotolerant and halophilic actinomycetes were isolated from sediment samples in three differentmedia viz. Starch casein agar (soluble starch 10 g/L; casein powder 1 g/L; sea water37 g/L; agar 15 g/L; pH 7.2 2), R2A agar (casein acid hydrolysate 0.5 g/L, yeastextract 0.5 g/L, proteose peptone 0.5 g/L; dextrose 0.5 g/L; soluble starch 0.5 g/L; dipotassiumphosphate 0.3 g/L; magnesium sulphate 0.024 g/L; sodium pyruvate0.3 g/L; pH 7.2 2) and Inorganic salt starch agar (soluble starch 10 g/L; di-potassiumphosphate 1 g/L; magnesium sulphate 1 g/L; ammonium sulphate 2 g/L; calciumcarbonate 2 g/L; ferrous sulphate 0.001 g/L; manganous chloride 0.001 g/L;agar 15 g/L; pH 7.2 2) at 4 different salt concentrations, 32, 50, 75 and 100 psu.
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